Plasmids are circular pieces of DNA found in bacterial cells that replicate independently from the host’s chromosome. They’re powerful and ubiquitous tools that touch nearly all areas of biological research. In general, plasmids are used to express specific genes in target cells, introduce new elements, and can be used in a variety of other genetic manipulations. In this section of the blog, learn more about the different parts of a plasmid, molecular cloning techniques, tips for using plasmids, specific plasmid-based tools, and more.
The Plasmids 101 blog series contains all the basics about plasmids. Many of the Plasmids 101 blog posts will also feature topics within the other sections described below so be sure to check both topics to find what you need.
With all the different cloning techniques out there, it can get overwhelming. This section of the blog focuses on different cloning methods and discusses some things to consider when choosing your cloning method.
Plasmids often carry many similar elements. In this section of the blog, find the details about each of these elements such as the restriction sites, antibiotic resistance genes, and the origin of replication.
Need some pointers for your experiments? This section has got you covered. While these aren’t necessarily step-by-step protocols (you can find that on the protocols page on Addgene’s website), these articles provide tips and tricks for common molecular biology methods.
Adding tags to proteins can help with many of your experiments. You can use tags for purification, stability, or solubility. Or you can use tags to monitor the localization of your proteins of interest.
Cre-lox can be used to induce site-specific recombination events and consists of a Cre recombinase and loxP recognition sites. Scientists have adapted these elements for cloning and for “flipping” the switch on gene expression.
Polymerase chain reaction (PCR) is a method that quickly makes millions of copies of a DNA sequence. PCR can be used in a variety of cloning procedures.
If you’re looking for specific plasmid for your research, this section is for you! Here, you’ll find our popular Hot Plasmids blog posts and blog posts featuring the work of Addgene depositors.