The Class 2 Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) system is an adaptive immune system in bacteria. It's been modified for genome engineering and used for many other applications in biology. From base editing, to prime editing, to RNA editing, there's a lot of ways that CRISPR can be used. Learn more!
Read our CRISPR 101 blog posts for the basics on CRISPR: the components of CRISPR, gRNAs, and much more!
CRISPR endonucleases, or Cas proteins, cut nucleic acids based on the guide RNA sequence. Cas proteins come in many forms and have been adapted for different functions such as cutting DNA or RNA. Read these blog posts to learn about different Cas proteins.
Base and prime editing are two CRISPR approaches that adapt CRISPR components to make precise changes in the genome. Learn about these incredibly useful technique and see how you could use them in your own research.
Aside from genome editing in basic research, CRISPR can has been adapted for many applications. Read more about these applications, such as using CRISPR as an antimicrobial or for editing genes associated with disease.
There are many ways to deliver Cas proteins and gRNAs into your desired cells ranging from delivering Cas9-gRNA ribonucleoproteins, packaging them in a viral particle, and more.
Pooled libraries are single preparations that each contain many different plasmids. Plasmids in a pooled library have the same backbone, but express or target different genes. CRISPR pooled libraries are packed in lenvirus and each vector contains an individual gRNA targeting a single gene. CRISPR libraries contain 3-6 gRNAs targeting each gene.
In these blog posts, find tips and tricks on protocols using CRISPR. These encompass anything from tips for first time CRISPR users, new methods, and ways to improve CRISPR editing in your experiment.
Didn’t see a CRISPR tool you were looking for in the above sections? This catch-all has the rest. This section also contains quarterly updates on new CRISPR plasmid tools available at Addgene.
Addgene is a nonprofit repository for plasmids, viral vectors, and antibodies developed by the global research community.
